From The Polyploidy Portal

Chromosome painting for plant biotechnology.

Kato A, Lamb JC, Albert PS, Danilova T, Han F, Gao Z, Findley S, Birchler JA.
Methods Mol Biol. 2011;701:67-96.

Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress. This chapter details the labeling procedures and chromosome preparation techniques used to produce high-quality FISH signals on somatic metaphase and meiotic pachytene spreads.[1]

  1. Kato A et al. (2011) Chromosome painting for plant biotechnology. Methods Mol Biol 701: 67-96 PubMed
Personal tools